Lipopolysaccharide as a pro-inflammatory stimulus during early gestion delays the development of the offspring in the rat.

SCHEFFER FRIDA; DE LA CRUZ BORTHIRY, FL; CAÑUMIL, VA; FRANCHI, AM; BELTRAME JS; RIBEIRO, ML

Congreso; X Virtual Meeting Of The Latin American Society Of Maternal-Fetal Interaction And Placenta (SLIMP) 2022; 2022

The origin of implantation failures is unknown in at least 50% of cases. One hypothesis holds that subclinical infections cause an imbalance in pro- vs anti-inflammatory responses, with severe consequences for pregnancy progress. Preeclamptic patients have an increased proinflammatory profile that correlates with defects in uterine artery remodeling and trophoblast migration. In this context, we postulate that vascular remodeling could be a target of pro-inflammatory situations since it is highly regulated by the trophoblast and by the decidual immune system. Previous results of this laboratory showed that one intraperitoneal injection of LPS 20 μg/mg in day 5 of gestation provokes an increase in the transversa length of the uterine and arcuate arteries on day 9 of gestation and larger implantation sites in a rat model.Methodology: Wistar female rats on day 6 of gestation received four consecutive intraperitoneal injections of lipopolysaccharide (LPS, 20 μg/mg in day 6 and 50 μg/mg in days 7, 8 and 9 of gestation) or vehicle. E. Coli LPS was used as a pro-inflammatory stimulus. Animals were euthanized on day 15 of gestation to study the ongoing vascular remodeling or the pregnancy was allowed to carry to term. In the last group there were made several studies in the offspring.Results: Our results indicate that LPS delays the appearance of fur (p<0.05) and dentition (p<0.05), the opening of the eyes (p<0.05) and the auditory pavilion (p<0.05). Nevertheless, LPS don’t produce an effect in the size of the litter, in the day of partitution, in the weight of the offspring, in the ano-genital distance or in the length of the head. In addition, in the rats euthanized at day 15 of gestation LPS don’t have an effect in the placental efficacy or in the weights of the placentas and the embryos.The coloration of the uterus and the uterine arteries and the arcuate arteries was more violent in rats that received LPS as treatment.Conclution:The dose of LPS used generates visible effects on the development of the offspring, although not in all the parameters analyzed. It is necessary to carry out studies on the length of the uterine and arcuate arteries in rats on day 15 of gestation and to test whether alterations in the development of the offspring are observed with a higher dose.