LEPTIN DOES NOT ENHANCE CELL-MEDIATED IMMUNE RESPONSES FOLLOWING MYCOBACTERIAL ANTIGEN STIMULATION

SANTUCCI, NATALIA; DIAZ, ARIANA; BIANCHI, ERNESTO; SPINELLI, SILVANA; DATTILIO, LUCIANO; BONGIOVANNI, BETTINA; DIDOLI, GRISELDA; BRANDAN, NADIA; NANNINI, LUIS; BAY, MARIA LUISA; BOTTASSO, OSCAR

INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE

INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D)

Lugar: Paris; Año: 2014 vol. 18 p. 981 - 987

1027-3719

Background: Tuberculosis is a consumptive infectious disease characterized by a profound immune endocrine-metabolic imbalance including a diminution in Leptin plasma levels. Leptin seems to be the link between nutritional status and the development of the immune response involved in pathogen clearance. Objective: To examine the effects of Leptin on the proliferation and IFNγ production by peripheral blood mononuclear cells from tuberculosis patients and healthy controls stimulated with irradiated and sonicated Mycobacterium tuberculosis plus Leptin treatment, or not. Since macrophages are key cells for the containment of Mycobacterium tuberculosis infection the effect of Leptin on the production of IL-1β and IL-1Ra by the monocytic cell line THP-1 was also studied. Results: Leptin diminished the proliferative capacity of PBMC upon mycobacterial stimulation and had no effect on IFNγ production. Also, IL-1β and IL-1Ra secretion by THP-1 cells was not modified by Leptin treatment. Present results do not avail a Leptin supportive therapy attempting to correct the immune imbalance during tuberculosis.