Effect of dietary fish oil and vitamin E on DNA damage in dogs undergoing training.

RISSO A; PELLEGRINO F; CORRADA Y; NICOLOFF N; SEOANE A; RELLING A E

Encuentro; Annual Meeting and Trade Show; 2017

Effect of dietary fish oil and vitamin E on DNA damage in dogs undergoing training The aim of this study was to assess the effect of dietary supplementation with fish oil (FO) alone or together with vitamin E (VE) on DNA damage in dogs undergoing training. Additionally, serum VE concentrations were evaluated. Using a replicate 3x3 Latin square design, six male dogs (2-6 yrs, 21-35 kg) were assigned to three groups: control diet (CG), CG supplemented with 54 mg FO/kg body weight0.75 (FG), and FG plus 400 mg VE (FEG) per day for 60 days. Blood samples were collected on days 0, 30 and 60. DNA damage was classified in four classes (0-I, undamaged; II, minimum damage; III, medium damage; IV, maximum damage). Values are expressed as the mean number of damaged nucleoids (sum of classes II, III, and IV) and the mean comet score for each treatment group. Furthermore, DNA damage index (DI) was calculated using the formula DI = [(I) + 2(II) + 3(III) + 4(IV)]/N(0--IV), where 0--IV represent the nucleoid type, and N0--NIV the total number of nucleoids scored. Dogs were trained on a treadmill with a 7.5% slope at 8 km/h twice a week for 60 days. Each session lasted 30 min. Data were analyzed using SAS 9.4. We investigated DNA damage by single cell gel electrophoresis in peripheral whole blood leukocytes of dogs undergoing training with or without dietary supplementation. At day 30, DI significantly increased in FG compared with CG and FEG (P