Effect of long-term fish oil supplementation on semen quality and testosterone concentrations in male dogs.

A RISSO; PELLEGRINO F; Y CORRADA; RELLING A E

International Journal Fertility and Sterility

Cell Journal

Año: 2016 vol. 10 p. 223 - 231

%DFNJURXQG 0DQLSXODWLQJWKHGLHWDU\IDWW\DFLG)$FRQWHQWFDQDOWHU)$SUR¿OHVRI UHSURGXFWLYHWLVVXHV1XPHURXVUHVHDUFKHUVKDYHHYDOXDWHGWKHHIIHFWRI¿VKRLO)2VXSplementation on reproductive characteristics in domestic animals, but reliable information concerning dietary FO effects on semen quality and testosterone concentrations in dogs has not been reported. Therefore, this study evaluated the effects of dietary FO on semen quality and serum testosterone concentrations in dogs. 0DWHULDOVDQG0HWKRGV In this cross-over experimental study, 5 male dogs consumed either a control diet or the same diet supplemented with 54 mg FO/kg metabolic body weight (BW) for 120 days. After the 120-day wash-out period, control (C) dogs received )2DQG)2IHGGRJVFRQVXPHGWKHFRQWUROGLHW,QWKH¿UVWSHULRGGRJVZHUHDOORFDWHG to the FO group and 3 to the C group. In the second period, 3 dogs were allocated to the FO group and 2 to the C group. Semen samples collected on days 0, 60, 90 and 120 were HYDOXDWHGE\VWDQGDUGPHWKRGV'D\VHPHQVDPSOHVZHUHDQDO\]HGIRU)$SUR¿OHV Blood samples were collected on days 0, 30, 60, 90 and 120 to measure serum testosterone concentrations. Data were analyzed by analysis of variance with repeated measures using the Mixed Models procedure of SAS (version 9.0, SAS Institute Inc., Cary, NC, 86$$QLPDOVDQGSHULRGRIWLPH¿UVWRUVHFRQGGD\VZHUHUDQGRPYDULDEOHVDQG WUHDWPHQWWLPHDQGWKHWUHDWPHQWE\WLPHLQWHUDFWLRQZHUHFRQVLGHUHG¿[HGHIIHFWV 5HVXOWV FO supplementation increased the percentage of motile sperm (P=0.02), total sperm count (P