Mononuclear immune cells distribution in the endometrium during postpartum of dairy cows

CAINELLI S.; PERALTA, M.B.; ANGELI, E.; STASSI A.F.; ORTEGA, H.H.; VELAZQUEZ M.M.L.

Mar del Plata

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Sociedad Argentina de Investigación Clínica

The processes involved in the resolution of uterine inflammationduring the postpartum period are closely related to an enhancedfertility during the subsequent lactational period. However, little isknown about the role of immune cell populations in the endometrialremodeling, restoration and clearance during bovine postpartum.The aim of this study was to analyze the endometrial profileof mononuclear immune populations in absence of disease duringbovine postpartum, and their possible relation with delayed conception.Endometrial biopsies were taken from twenty multiparous Holsteindairy cows (second to four lactation, at 45 and 60 days in milk(DIM). All dairy cows returned to their normal cycles before 45 DIM.Their voluntary waiting period was 70 DIM and after one they receivedthe first insemination. Histological sections from endometrialbiopsies were processed to analyze the cell immunolocation of totalT lymphocytes (LT), Tγδ lymphocytes (LTγδ), B lymphocytes (LB)and macrophages (MØ) by indirect immunohistochemistry (IHC).Dairy cows were grouped according to conception parturition interval(CPI) for comparison: CPI fewer than or equal 90 DIM (CPI≤90),CPI between 90 and 120 DIM (CPI90-120) and CPI greater than 170DIM (CPI≥170). A Generalized Linear Model (GLM) was used to analyzethe mononuclear immune cells distribution in the endometrium.Immunostained cells were mainly located in the endometrial stroma.The number of MØ and LB per mm2 at 45 DIM between the groupsshowed significant differences (P