Altered chemotaxis and mononuclear cell infiltration in bovine ovary after acth stimulation.

ETCHEVERS L; STASSI A.F.; BELOTTI, E.M.; CABAÑA, E; ANGELI, E.; DÍAZ, P.U.; REY, F.; SALVETTI, N.R.; ORTEGA, H.H.; AMWEG, A.N.

Congreso; LXVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2021

Before ovulation the ovary exhibits signs of local inflammation:chemokine and cytokine release, vasodilation, leukocyte infiltration;and altered immune responses in ovary often results in reproductivefailures. However, the effects of adrenocorticotropin (ACTH) on thecomplexity of this inflammatory response are not yet well described.The aim of this study was to evaluate the effect of ACTH duringpreovulatory period on the number of mononuclear cells (MCs) infiltrated in the ovary and on the mechanisms associated with cellinfiltration. ACTH (100 IU) was administered to Holstein cows (n=11)during proestrus every 12 h for four days before ovulation when ovariectomy was performed (day 18). Daily ovarian Doppler ultrasonography was used to evaluate the percentage of irrigated area (IA), thepulsatility and resistance index on the dominant follicles. Also, bloodsample was taken on day 18 to analyze alterations on circulatingMCs using an hematology analyzer. Macrophages (CD14), T (CD2)and B (CD79) lymphocytes distribution in ovary was analyzed by immunohistochemistry. In follicular wall samples, gene expressionof chemokines MCP1, IL8, CXCL1, CCL25 were evaluated. And,IL17a expression was analyzed by western blot. A Generalized Linear Model was used to analyze the results. The number of systemicmonocytes and lymphocytes were similar between groups (p>0.05).However, the total number of CD14, CD79 and CD2 infiltrated cellswere lower in ACTH group than in control group (p