Ovarian mononuclear cells distribution and its influence on pathogenesis of bovine cystic ovarian disease (COD)

STASSI A.F.; ETCHEVERS L; CAINELLI S.; AMWEG, A.N.; ORTEGA, H.H.; REY, F.; SALVETTI, N.R.

Congreso; LXVI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2021

COD results from failure in the ovulation and the study of the processes that lead to this failure and persistence of the dominant follicle in the ovary is the key to understand the pathogenesis of CODin cattle. Ovulation has been characterized as an inflammatory process and mononuclear cells (MC) participate in this process, therefore, the aim of this study was to evaluate the populations of macrophages (CD14+), T (CD2+) and B (CD79+) lymphocytes in ovariesof animals with induced follicular persistence and spontaneous COD(sCOD). Ovariectomy was performed to obtain ovaries with sCOD(n = 5). Also, an experimental model of follicular persistence wasperformed, with an intravaginal progesterone (P4) device to getsubluteal concentrations of P4, obtaining dominant follicles aroundovulation (n = 5; P0) and follicles that persist for 5 (n = 5; P 5), 10(n = 5; P10) or 15 days (n = 5; P15) after the expected time of ovulation. Controls cows were ovariectomized in proestrus (n = 5; C).MC populations were evaluated through immunohistochemistry inovarian cortex, medulla, theca interna and externa of persistent follicles, cysts and dominant. The specificity of the antibodies was corroborated by western blot. The data were analyzed through ANOVAwith Duncan post-test to contrast the evaluated structures of the experimental model and C group, and T-student test for sCOD and Cgroup. The number of CD14+, CD2+ and CD79+ cells was higher inovarian cortex, medulla, theca interna and externa of C group thanin sCOD, P0, P10 and P15 groups (p0.05). Because these populations constitute potential insitu modulators of ovarian function, acting through the secretion ofregulatory factors of the inflammatory process related to ovulation,we propose that this low proportion of these cells could be partlyresponsible for the anovulation observed in COD.