"Identification of the miRNAome of early mesoderm progenitor cells and cardiomyocytes derived from human pluripotent stem cells"

GARATE XIMENA; LA GRECA ALEJANDRO; NEIMAN GABRIEL; BLUGUERMANN CAROLINA; NATALIA LUCÍA SANTÍN VELAZQUE; LUCÍA NATALIA MORO; LUZZANI CARLOS; SACSSA MARIA ELIDA; SEVLEVER GUSTAVO EMILIO; ROMORINI LEONARDO; MIRIUKA SANTIAGO GABRIEL

Scientific Reports

Nature Publishing group

Año: 2018

MicroRNAs are small non-coding RNAs involved in post-transcriptional regulation of gene expression related to many cellular functions. We perform a small-RNAseq analysis of cardiac differentiation from pluripotent stem cells. Our analyses identi1ed some aspect about microRNA expression in this context. First, we described a dynamic expression pro1le of microRNAs in where some of them are clustered according to their expression behavior. Second, we described the extensive network of isomiRs and ADAR modi1cations. Third, we identi1ed the microRNAs families and clusters involved in the establishment of cardiac lineage and imply that the mirRNAome can be linked to this grouping. Finally, we were able to de1ne a miRNAome associated with cardiomyocytes by comparing the expressed microRNAs with other mature cells. MicroRNAs exert their effect in a complex and interconnected way, making necessary a global analysis to better understand their role. Our data expands the knowledge of microRNAs and their implications in cardiomyogenesis.