INVESTIGADORES
BLUGUERMANN Carolina
artículos
Título:
Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts
Autor/es:
QUESTA MARÍA; ROMORINI LEONARDO; BLUGUERMANN CAROLINA; SOLARI CLAUDIA; NEIMAN GABRIEL; LUZZANI CARLOS; SCASSA MARÍA ELIDA; SEVLEVER GUSTAVO EMILIO; GUBERMANN ALEJANDRA; MIRIUKA SANTIAGO GABRIEL
Revista:
STEM CELL RESEARCH
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 16 p. 300 - 303
ISSN:
1873-5061
Resumen:
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls.
