Rsb and FST analysis revealed selection swifts in hypoxia-inducible factor genomic region on Bolivian highland Creole cattle

ALVAREZ CECCO, P; FALOMIR LOCKHART AH; PEREIRA RICO JA; LOZA VEGA A; ARCE CABRERA ON; FERNANDEZ ME; ROGBERG MUÑOZ A; GIOVAMBATTISTA G

Mendoza

Congreso; XVII Congreso Latinoamericano de Genética; 2019

Asociación Latinoamericana de Genética

Latin-American Creole cattle are spread all over American countries and are descendants of the bovines introduced into America by the Spanish and Portuguese conquerors in the 16th century. Most of populations evolved under low levels of breeding management and became adapted to different environments, such as tropical rainforest, subtropical dry forest, highland steeps. In Bolivia, some Creole populations have adapted to the Andean highland plains at 4000 m altitude, which probably created signatures of selection within the actual genomes. The aim of this study was to study the footprints of adaptation to altitude in a population of Bolivian Creole cattle. Hence, 132 animals (67 from highland and 65 from lowland plain) were genotyped using ArBos1 50K microarray. Quality control was performed considering 97% as sample and SNP call rates and MAF values. Shape-IT software was used to determine the individual haplotypes and the rehh package from R was applied to compute Rsb. Plink v1.9 was used to estimate pairwise FST between populations. The top 1% values from both indexes were taken as threshold to consider significant results and BovineMine database was used to retrieve the genes located in those regions. The most significant results were found on chromosomes 10 and 20, which showed significant peaks through both methods. Within BTA10 we detected a gene, HIF1 (hypoxia inducible factor 1), that functions as transcriptional regulator of the adaptive response to hypoxia and was previously associated to altitude adaptation in several species. Further analysis will be done to increase the understanding of cattle adaptation to high-altitude environments.