Chlorpyrifos-induced molecular mechanisms involved in breast cancer progression

LASAGNA M; HIELPOS, M.S. ; VENTURA C; M MARDIROSIAN; MARTIN G; MIRET, N.; A RANDI; M NUÑEZ; COCCA C

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR; 2021

SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC)

Chlorpyrifos (CPF) is one of the most widely used pesticides due to the low cost, high effectiveness, and broad spectrum. CPF is currently considered an Endocrine Disruptor (ED). EDs have been associated with the progression and metastasis of established breast tumors and were pointed out as potential inductors of the epithelial mesenchymal transition (EMT). In this study, we investigate whether CPF promotes molecular mechanisms involved in breast cancer progression. Our experiments were performed using MCF-7 and MDA-MB-231 cell lines, and two CPF concentrations (0.05 and 50 μM). We evaluated the migration and invasion using 2D and 3D models, as well as the expression of EMT biomarkers. We show that 50 μM CFP induces invasion in MCF-7 and MDA-MB-231 cells (p