The Human Voltage-Activated H+ Channel (hHv1) is Highly Expressed in Acute Myeloid Leukemia and Correlated with the Monocytic Differentiation Stage.

ISSOURIBEHERE D; FINOCHIETTO P; VENTURA C; JUAN IGNACIO FELICE; ENRIQUE N; VELLI C; MILESI V

Ciudad Autónoma de Buenos Aires

Congreso; VI International Congress in Translational Medicine.; 2023

Facultad de Farmacia y Bioquímica, UBA

Background: The human voltage-activated H+ channel (hHv1), codified by the HVCN1 gene, is an extremely selective protein which mediates passive H+ outward currents activated by different stimuli. This channel has important roles in the immune system cells. It was reported that a H+ conductance appeared and increased during the differentiation process in myeloid tumoral cells. So, we hypothesize that the hHv1 channel could be responsible for that conductance and, in this way, a marker of myeloid cell differentiation. Taking into account that the FAB (Franco-American-British Cooperative Group) classification of acute myeloid leukemias (AML) is based on the differentiation stage of pathological cells we investigated if the expression of hHv1 correlated with the FAB subtypes. Methods: we analyzed the expression profile of HVCN1 (at mRNA level) in AML human oncological samples (TCGA) using data available in public datasets [GEPIA 2 and cBioPortal]. hHv1 protein expression was measured by flow cytometry in human bone marrow samples from control and AML-diagnosed patients in the Hospital El Cruce, Argentina using specific antibodies. Results: Using GEPIA2 public database we found that HVCN1 gene expression is up-regulated in AML human samples compared to control (Student’s t-test, p