EXPRESSION AND FUNCTION OF THE HV1 PROTON CHANNEL IN HUMAN T LYMPHOMAS CELL LINES

FINOCHIETTO P; ISSOURIBEHERE D; CAYROL F; DEBERNARFI M; CREMASCHI G; MILESI V; VENTURA C

Mar del Plata

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Sociedad Argentina de Investigación Clinica (SAIC)

The Hv1 channel is a membrane protein present in the immune cells which allows the passive extrusion of H+. In human B type leukemic cells, it was reported that its short, more active, isoform is associated with an increased cell proliferation. Then, we showed that in Jurkat cells, derived from a human T-cell acute leukemia, Hv1 inhibition induces apoptosis associated with a diminution of intracellular pH (pHi). Objectives: to expand the knowledge about the role of Hv1 in malignant T cells, we have explored two cell lines derived from human T lymphomas: HuT78 and K299. Methods and Results: by patch clamp technique, we observed that only HuT78 cells evoked H+ currents mediated by Hv1 (65.8 ± 15.1 pA, p