VOLTAGE-GATED PROTON CHANNEL OVEREXPRESSION IN HUMAN BIOPSY-DERIVED ACUTE MYELOID LEUKEMIA BLAST CELLS

ISSOURIBEHERE D; ENRIQUE N; VENTURA C; MARTIN P; MONCADA M; LOUDET SM; BORDONE J; SCANDIZZO, HILDA EMILIA; MILESI V

Mar del Plata

Congreso; LXVII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2022

Sociedad Argentina de Investigación Clinica (SAIC)

Some tumor cells change their energy metabolism towards glycolytic pathways, which end up generating a higher concentration of acid species. The voltage-gated proton channel (Hv1) is a membrane protein capable of mediating H+ efflux. There is a long and a truncated isoform, and the truncated isoform generates greater extrusion of H+. Objectives: Our goal was to assess the expression of long isoform and total expression of the Hv1 channel in blast cells derived from acute myeloid leukemia (AML). Methodology: In collaboration with the Flow Cytometry Service of the El Cruce Hospital, we worked with bone marrow samples from patients for diagnosis ofAML. We labeled the cells with two antibodies: Anti-Hv1L, which recognizes an epitope present only in the long isoform, and Anti-Hv1T, which recognizes an epitope present in both Hv1 isoforms. Flowcytometry was used to quantify the median fluorescence intensity of the antibodies and the values obtained in the pathological cells were compared with the values of the non-pathological cells of the same lineage. We had 3 bone marrow samples from patients with a negative diagnosis used as control, and 6 samples from patients diagnosed with different subtypes of AML. We evaluated monocytic and granulocytic differentiated cells. Results: In monocytes, the Anti-Hv1L intensity was 2.796 ± 598 in control cells vs 10.802 ± 2.377 in pathological cells (p