Comparison of two protocols for field immobilization of white-eared opossums (Didelphis albiventris)

WAXMAN S; OROZCO MM; ARGIBAY H.; RODRÍGUEZ C; OTERO, P.

EUROPEAN JOURNAL OF WILDLIFE RESEARCH

SPRINGER

Lugar: Berlin; Año: 2018 vol. 64

1612-4642

The aim of this study was to investigate the efficacy of two protocols for field immobilization of white-eared opossums (Didelphis albiventris) and compare their effects on immobilization, cardiopulmonary variables and recovery times. Twenty one opossums were randomly divided into two groups; G1 received ketamine (15 mg kg-1)-dexmedetomidine (0.15 mg kg-1) intramuscularly (IM), G2 received the ketamine-dexmedetomidine combination and isoflurane once induction was achieved. Oxygen was delivered by face mask (1.5 L minute-1), Thirty minutes after induction, isoflurane was discontinued (G2) and both groups were administered atipamezole (1.5 mg kg-1) IM. Respiratory (?R) and heart rate (HR), oxyhemoglobin saturation (SpO2) and rectal temperature (T) were recorded every 5 minutes. Induction time, time to first movement (RT1) and to achieve standing (RT2) were recorded. ANOVA and non-parametric tests were used. Level of immobilization was assessed by observation of movements and evaluation of muscle relaxation. Mean induction time was 4.71 minutes. RT1 and RT2 were significantly longer in G2. No significant differences were found in SpO2 or ?R. HR did not vary significantly along time, but was higher in G2. Rectal temperature did not show differences between treatments, but decreased significantly with time in G2. Four of nine animals in G1 showed movements, muscle relaxation was determined to be better in G2. Both protocols were adequate for short-term field immobilization, with minimal alterations of HR and T and relatively short recovery times. Isoflurane provided better immobilization with statistically significant prolongation of recovery times.