Proliferation and cytokine profile of T. annulata-infected ovine, caprine, and bovine lymphoblastoid cells.

SCHNITTGER L, HOLLMANN C, DIEMER U, BOGUSLAWSKI K, AHMED JS

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES.

Año: 2000 p. 676 - 680

0077-8923

T. annulata, the causative agent of tropical theileriosis in cattle, can also infect ovine and caprine leukocytes in vitro. In vivo studies showed that this parasite causes a mild infection in both these animal species, and in sheep merozoite stage development seems to be inhibited. Since the nature of T. annulata infected caprine and ovine cells is not known, all three cell lines were caryotyped and phenotypically characterized by flow cytometry. They all express mRNA of cytokines IL-1alpha, IL-1beta, IL-8, and TNF-alpha, but not of IFN-gamma, IL-2, and IL-4. In contrast, IL-6 mRNA was expressed in the cattle cell line only, while mRNA of IL-10 was exclusively produced by the sheep cell line. The observed differences in cytokine mRNA expression may be responsible for the different pathogenesis of T. annulata infection in cattle and sheep.