INVESTIGADORES
SCHNITTGER Leonhard
artículos
Título:
Simultaneous detection and differentiation of Theileria and Babesia parasites infecting small ruminants by reverse line blotting.
Autor/es:
SCHNITTGER L, YIN H, QI B, GUBBELS MJ, BEYER D, NIEMANN S, JONGEJAN F, AHMED JS.
Revista:
PARASITOLOGY RESEARCH
Referencias:
Año: 2004 p. 189 - 196
ISSN:
0932-0113
Resumen:
Characteristic sequence signatures were identified within the
hypervariable region 4 (V4 region) of the small ribosomal RNA gene of
ovine/caprine piroplasm species including Theileria lestoquardi, T.
ovis, T. separata, Babesia ovis, B. motasi, B. crassa [comprising
strains B. crassa (Iran) and B. crassa (Turkey)] and several novel
species: Theileria sp. 1 (China), Theileria sp. 2 (China) and Babesia
sp. (China), [comprising strain Babesia sp. (Lintan), and Babesia sp.
(Ningxian)] as defined previously. Based on the ascertained gene
variations a reverse line blotting (RLB) assay was developed enabling
direct, concurrent, highly specific and sensitive identification of
virtually all presently known ovine/caprine piroplasm species. All
probes bound to their respective target sequence only, therefore, no
cross-reaction was observed resulting in clear recognition of either
individual strains, species or groups. No signal was observed when
ovine and caprine genomic DNA was used as the control, demonstrating
that the signals are due to the presence of parasite DNA in
investigated samples. Furthermore, the sensitivity of RLB could be
considerably enhanced to detect a parasitemia level of at least
10(-12)% by reamplification of PCR products (nested PCR) thereby
substantially increasing the possibility of identifying carrier animals.