INVESTIGADORES
SCHNITTGER Leonhard
artículos
Título:
Ribosomal small-subunit RNA gene-sequence analysis of Theileria lestoquardi and a Theileria species highly pathogenic for small ruminants in China.
Autor/es:
SCHNITTGER L, YIN H, JIANXUN L, LUDWIG W, SHAYAN P, RAHBARI S, VOSS-HOLTMANN A, AHMED JS.
Revista:
PARASITOLOGY RESEARCH
Referencias:
Año: 2000 p. 352 - 358
ISSN:
0932-0113
Resumen:
A fatal disease of sheep and goats in the northwestern part of China
has been reported to be due to Theileria lestoquardi (syn. T. hirci).
However, some characteristics of the causative agent are not in
accordance with attributes ascribed to this parasite. We therefore
determined the nucleotide sequence of the small-subunit ribosomal RNA
(srRNA) gene of T. lestoquardi and the parasite identified in China and
compared it with that of other Theileria and Babesia species. In the
inferred phylogenetic tree the srRNA sequence of the Chinese parasite
was found to be most closely related to T. buffeli and clearly
divergent from T. lestoquardi, suggesting that it is an as yet
unrecognized Theileria species. Extensive structural similarities were
observed between the srRNA sequences of T. lestoquardi and T. annulata,
revealing a close phylogenetic relationship between these two Theileria
species. On the basis of the srRNA nucleotide sequence, polymerase
chain reaction (PCR) primers were designed that specifically amplified
genomic DNA of the Chinese Theileria species. These primers may be
valuable tools in future epidemiology studies.