From molecule to diagnostic tool: Theileria annulata surface protein TaSP

ULRIKE SEITZER, MOHAMMED A. BAKHEIT, DIA ELDIN A. SALIH, AWADIA ALI, DANIEL HALLER, HONG YIN, LEONHARD SCHNITTGER, JABBAR S. AHMED

PARASITOLOGY RESEARCH

Año: 2007 p. 217 - 223

0932-0113

Isolation and characterization of the Theileria annulata macroschizont stage protein TaSP showed that this parasite surface membrane proteins is a highly antigenic protein suitable for the development of diagnostic tools for tropical theileriosis. An enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antibodies against Theileria annulata was established and validated using the recombinantly expressed TaSP protein. The ELISA has subsequently been applied for cross-sectional surveys to determine the distribution and prevalence of tropical theileriosis in Sudan.