Estudio histológico e histoquímico de las glándulas anexas del tubo digestivo de juveniles de pejerrey (Odontesthes bonariensis).

MÁNES, J.; AGELET, L.; VIGLIANO, F.A.; CERUTTI, P.

Rosario, Argentina

Congreso; V Congreso y XXII Reunión Anual de la Sociedad de Biología de Rosario; 2002

Sociedad de Biología de Rosario

In this study, the histological structure of appended glands of the digestive tract in juvenile of Odontesthes bonariensis is determined. Samples from fifty specimens were taken, fixed in 10% formaldehyde and embedded in paraffin. Sections of 2 μm thickness were stained with HE, P.A.S., Orcein Acid-Giemsa, Prussian Blue Stain and Gallego Trichrome. Results showed that the liver presented a thin capsule of connective tissue covered with serosa. Hepatocytes appeared separated by capillaries and radially distributed from central veins. Liver cells had a polyhedral shape, a large nucleus centrally located, lax chromatin and an evident nucleolus. Its cytoplasm showed a perinuclear zone intensely basophilic, P.A.S. positive and a marginal region with lipid vacuoles. Among hepatocytes small ducts formed by a simple cubic epithelium were observed. Prussian Blue reaction did not show a high activity. The pancreas possessed two portions, an intrahepatic one and extrahepatic one. Both portions were represented by pancreatic acini. The intrahepatic portion was formed by acinic nodules. These acinic groups were distributed around a central blood vessel. The extrahepatic portion had a lobular distribution. Acinar cells showed a pyramidal shape. The nucleus, basally located, had lax chromatin and a prominent nucleolus. A large quantity of acidophilic and apically located granules of secretion were seen. The structures of appended glands of the digestive tract of Odontesthes bonariensis here described are in agreement with thosedescribed for other teleosts.