Whole genome sequencing of P. brasiliensis isolates of endemic areas in Argentina and Paraguay

CATTANA ME; TEIXEIRA M; TRACOGNA F; ARECHAVALA A; NEGRONI R; SANTISO G; CECH N; ORTELLADO J; CHACON Y; NIÑO-VEGA G; ALVARADO P; BARRETO L; SOSA MA; ROJAS F; BARKER BM; GIUSIANO G

Amsterdam

Congreso; 20th Congress of the International Society for Human and Animal Mycology; 2018

International Society for Human and Animal Mycology

Objective: This study aims to describe clinical and molecular epidemiological aspects of PCM cases from Argentina and Paraguay, to analyze the geographical origin and to compare the genetic profile versus Paracoccidioides species circulating in other countries.Methods: Using whole-genome sequencing typing the genetic profile of 21 Paracoccidioides isolates from Argentina (15) and Paraguay (6) were sequenced ?de novo? and analyzed. Additionally, 12 P. brasiliensis strains from Venezuela (7), Brazil (2), Bolivia (1), Colombia (1), and Peru (1) were included in the dataset.Results: Table 1 presents clinical and molecular characteristics of the 33 Paracoccidioides isolates included in this study.Figure 1 shows the Maximum Likelihood tree generated by whole genome SNP typing.Conclusion: Phylogenomics analysis suggests that two different P. brasiliensis genotypes are endemic in Argentina: P. brasiliensisS1a and S1b. In contrast, Paraguayan isolates clustered only with S1b. Comparing the genetic profile obtained with ones published by Mu? noz et al. (2016) we observed: 1) isolates clustered within P. brasiliensis S1b are close related with the reference strain Pb18 and S1b isolates widely distributed in Brazil. 2) Analyzing the genetic cluster composed by P. brasiliensis S1a lineage, Argentinian isolates and two from Mato-Grosso state (Brazil) form a monophyletic clade that is separated from S?ao Paulo state isolates (Brazil) showing a high level of geographic relationship within this genetic cluster. Although further studies are required to conclude, all P. brasiliensis S1a identified were related to chronic forms of PCM. Possibly there is not only a geographic relationship, but also some relationship with the clinical form of PCM.Picture 1: https://www.eventure-online.com/parthen-uploads/89/8ISH/add 1 422379 05055166-39d5-44cf-8f19-d884aa206 bf3.pngCaption 1: Figure 1 - Maximum Likelihood tree generated by whole genome SNP typing showing the main genetic entities of Paracoccidioides reported so far.Picture 2: https://www.eventure-online.com/parthen-uploads/89/8ISH/add 422379 05055166-39d5-44cf-8f19-d884aa206 bf3.pngCaption 2: Table 1 - Clinical and molecular characteristics of the 33 Paracoccidioides isolates included in this study