Defining the optimal time to perform ICSI: effect of oocyte in vitro maturation interval on embryo quality and development.

RODRIGUEZ, MB; CLERICO, G.; GAMBINI, A; RODRIGUES, D; TAMINELLI, G; VERONESI, JC; FERNANDEZ, S; SANSINENA, M; SALAMONE D

Cambridge

Simposio; XII International Symposium of Equine Reproduction; 2018

ISER / Churchill College.

The request of in vitro equine embryo production by ICSI has increased in clinical reproduction over the last years. However, the efficiency of this technique remains variable with reported blastocyst rates between 10 ad 41%. Oocytes recovered by transvaginal follicular aspiration from donor mares are valuable and limited, and they are often collected from immature follicles. These oocytes require further in vitro maturation in order to be competent to be fertilized and reach the blastocyst stage. For this reason, it is crucial to determine when is the optimal time to perform ICSI after oocyte maturation started. Different in vitro maturation intervals have been reported to produce ICSI embryos in horses (28-30h[1]; 36 h-[2]; 38 h [3]; 20-22 h- [4]). However, there is no consensus about the optimal maturation interval to perform ICSI and to produce high quality embryos. An important aspect of the preimplantation embryo development involves events of cell differentiation, with the formation of the inner cell mass (ICM) and the trophectoderm (TE). The Hippo signaling pathway has been shown to play an important role in mice and cattle [5,6], but it has not been described in horses to date. Nuclear localization of a protein called Yes-associated protein 1 (YAP1) in TE cells affects the expression of CDX2 and subsequent cell differentiation. YAP1 functions as a critical co-activator for TE development in the nucleus of TE cells, but in the inner cell mass, it stays mainly in the cytoplasm as a phosphorylated inactive form [6]. In this work, we aimed to evaluate developmental rates and quality of ICSI embryos produced from oocytes matured for two different time intervals: I) 22 to 24 h and II) 28 to 30 h. Blastocyst development and size were recorded. Embryo quality was assesed by analysis of apoptotic cells (TUNEL assay), and expression and distribution of YAP-1 by immunoflouresce (IF).