Pregnancy rates after repeated transvaginal ultrasound-guided aspirations performed in buffalo cows in Argentina

KONRAD, J; GARRIDO, MJ; YUPONI, R; YUPONI, M; CRUDELI, G; SANSINENA, M

Cartagena de Indias

Congreso; XI World Buffalo Congress; 2016

WBA

Introducción y Objetivos. The ovum pick up (OPU) is an alternative technique for harvesting high quality buffalo oocytes for in vitro fertilization and in vitro embryo production. Despite of several research reporting that OPU technique is save and repeatable, there are not much information about the effect of repeated puncture above buffalo cow fertility in long term. The aim of this work was to assess if repeated OPU applied in buffalo cows affects pregnancy rates.Materiales y métodos. The trial was carried out on twelve healthy Buffalo cows, and OPU was performed once (n=4) or five times (n=8) during the breeding season. Fixed-time artificial insemination or natural service was performed after the last OPU. Pregnancy diagnosis was performed by ultrasound, and number of days since the last OPU to pregnancy was estimated. Categorical data were analyzed by GENMOD procedure and continuous data by MIXED procedure of SAS.Resultados. An overall pregnancy rate of 83.3% (10/12) was obtained. The treatment of one OPU showed a pregnancy rate of 100% (4/4), whereas five OPU treatment reach 75% (6/8). No significant differences were found between the two treatments (p>0.05). Those buffalo cows that received one OPU conceived 46.25±16.41, whereas buffalo cows that received five OPU got pregnancy after 66.67±13.4 days after the last OPU. No significant differences were found between the two treatments (p>0.05).Conclusiones. Oocytes harvesting by OPU technique does not affect pregnancy rate of buffalo cows.