Histamine-treated dendritic cells improves recruitment of type 2 CD8 T cells in lungs of allergic mice

MARIA MARTA AMARAL; CAROLINA ALVAREZ; JORGE GEFFNER; MÓNICA VERMEULEN

IMMUNOLOGY

Wiley-Blackwell

Lugar: Londres; Año: 2010 vol. 130 p. 589 - 596

0019-2805

Histamine controls the function of dendritic cells (DC). It appears to be required for the normal development of DCs. It also induces the chemotaxis of immature DC and promotes the differentiation of T CD4+ cells into a Th2 profile. Moreover, we have recently shown that histamine stimulates both the uptake and the cross-presentation of antigens by DC, supporting that histamine might favor the activation of TCD8+ cells during the development of allergic pathologies. Here, we analyzed whether the course of allergic response, in a well-defined model of ovalbumin (OVA)-induced allergic airway inflammation, could be modulated by intratracheal injection of OVA-pulsed DC previously treated with histamine (DCHIS). Compared with control DC, DCHIS induced: a) a higher recruitment of T CD8+ cells in the lung, b) a higher stimulation of the production of IL-5 by lung T CD8+ cells, and c) an increased recruitment of CD11c/CD8 double positive DC in the lungs of allergic mice. Moreover, mice-treated with DCHIS showed increased levels of serum-specific IgE antibodies directed to OVA, and a more persistent presence of eosinophils in BALF compared with mice treated with OVA-pulsed control DC. Our results support the notion that histamine, by acting on DC, promotes the severity of allergic processes.