“SERPIN 1F: DETECTION ON RAT TISSUES BY RT-PCR”.

MONCLUS MA; SAEZ LANZELLOTTI TANIA ESTEFANIA; CAYADO N; PIETOBON EO; VINCENTI A.E.; FORNES MW

Congreso; XXX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

In many mammals’ species during their epididymal journey, sperm associated by their heads when they reach the Cauda, but not in the proximal regions of this organ. These associations, called Rosettes were previously characterized in Mouse and Rat. This suggests a maturational process, probably due to the milieu that contains several proteins secreted by the caudal epithelium. The physiological meaning of this behavior remains unclear. By chromatographic techniques we previously isolate and identified SERPIN 1F, a member of the Serpins family (Serin proteases inhibitors). This protein described by the first time in rat was involved in the sperm re association during in vitro assays. We isolate RNA from different epididymal regions and by RT-PCR the presence of the Serpin 1F mRNA was observed in the samples analyzed.