Mouse Sperm Association: Role of Ą-D-Glucose y/o Ą-D-Manose

MONCLUS MA; CABRILLANA ME; VINCENTI AE; BURGOS MH; FORNES MW

Mendoza, Argentina

Congreso; Reunión Conjunta de: XXI Reunión Científica Anual de la Sociedad de Biología de Cuyo, 10¢X Aniversario de BIOCELL y Reunión Fundacional de la Sociedad Argentina de Microscopía; 2003

Sociedad de Biología de Cuyo

Mouse sperm association: role of a-D glucose and a-D mannose Monclus MA, Cabrillana ME, Vincenti AE, Burgos MH and Fornés MW During the epididymal transit, sperm agglutinate forming rosettes. I fan epididymal fluid drop is placed in a culture mediums the sperm remains aggregated (rosettes) until disassemble in motile cells. This work Hill describe the rosettes morphology, the place of appearance, the transient character of the agglutination and the participation of  a-D glucose and a-D mannose in this assembling using either FITC or colloidal gold conjugated with concannavalin A. Drops of epididymal fluid were obtained by puncture and placed in Ringer buffer at 37ªC. Rosettes were checked out and fixed (4% picric acid- paraformaldehide). Rosettes were harvested and analyzed by light and transmission scanning electron microscopy. Rosettes dissasemblage was recorded by video microscopy. In other Mouse epididymal unilateral  ligatures were placed at the epididymis between   caput and corpus and the same analyses were performed 50 days later. Rosettes are composed by alive sperm, transitory associated by their heads and joined a concannavalin A positive material. Ultra structural observations revealed an electron dense material between heads. Alter 50 days of ligature rosettes were not retrieved from caput epididymal fluid. We suggest that rosettes are assembling during the epididymal trip with an electron dense material probably containing   a-D glucose and a-D mannose.