SERPIN F1 DIMINISHES MURINE SPERM ACTIVATION DURING IN VITRO ASSAYS

CONTE, MARÍA INÉS; ARANCIBIA CABAÑEZ MR; BASAEZ ALONSO MJ; LEPORATI JL; LOPEZ MARIA ELIS; SAEZ LANCELLOTTI TANIA ESTEFANÍA; FORNÉS MIGUEL WALTER; AGUILERA MERLO CLAUDIA; MONCLUS MARÍA DE LOS ÁNGELES

Congreso; XL Reunion Anual de la Sociedad de Biologia de Cuyo; 2022

For proper fertilization, mammalian sperm need to suffer different steps after ejaculation inside the female tract -capacitation, acrosomereaction (AR) and hyperactivation- considered together as a sperm activation. These processes are regulated by the loss of male“decapacitating” factors and the influence of female factors presents inside the female genital tract. A male decapacitating factor is a spermhead membrane molecule that allows capacitation after been removed such as SERPIN F1. This protein has been described recently by ourgroup. It is expressed in androgen-dependent manner in Wistar rat male´s reproductive tract. We have initiated the study with SERPINF1 as a possible decapacitating factor in murine sperm. We preincubated in vitro mouse epidydimal sperm with the anti-SERPIN F1antibody (to block the endogen protein) followed by adding different concentrations of recombinant SERPIN F1 (100, 500 and 1000 nM)- as exogen source. Then, we performed capacitation and AR triggered by calcium ionophore. We evaluated the AR by Coomasie Bluestain, and we noted a decrease in the percentage of AR with increasing concentrations of added Serpin. We also evaluated the AR triggeredby Progesterone using as experimental condition SERPIN F1 at 500 nM and the antibody, obtaining similar results. In other set ofexperiments, we incubated capacitated sperm with the antibody and the recombinant SERPIN F1 prior to AR stimulation with Progesterone(to distinguish pre or postcapacitation effect), obtaining a diminished percentage of AR. These results suggest that SERPIN F1 may affectthe sperm activation (measured as percentage of AR) both before and after in vitro capacitation of mouse spermatozoa.