EFFECT OF HIGH-FAT DIET ON SPERM QUALITY EVALUATED BY HETEROLOGOUS BINDING ASSAY OF RABBIT SPERMATOZOA TO ZP-FREE MOUSE OOCYTES

AVENA, MARÍA VIRGINIA; FUNES, ABI KARENINA; WETTEN PA; KLINSKY, OG; MONCLUS MARÍA DE LOS ÁNGELES; BOARELLI PAOLA VANINA; SAEZ LANCELLOTTI TANIA ESTEFANÍA; MICHAUT MARCELA; FORNÉS M.W.

Congreso; XL Reunion Anual de la Sociedad de Biologia de Cuyo; 2022

2024- HYPERTHYROIDISM ALTERS MAMMARY GLAND DEVELOPMENT DURINGLACTATION AND IMPAIRS OFFSPRING NUTRITIONSánchez MB1,2, Michel MC1,2, Viruel LB2, Neira FJ1,2, Rodríguez-Camejo C3, Pietrobon EO1,4, Soaje M1,5, Hernández A3, Jahn GA1,Valdez SR1,6, Mackern-Oberti JP1,5.1IMBECU; 2UMaza; 3UdeLAR; 4IHEM; 5UNCuyo FCM; 6UNCuyo, FCEN.E-mail: bsanchez@mendoza-conicet.gob.arMaternal milk is a great source of bioactive components, immune factors, hormones, and nutrients which are vital for the development ofthe newborns. Hyperthyroidism (H) alters maternal behavior and disrupts milk production. However, it remains unclear if H could generatechanges in the nutritional and immunological composition of maternal milk. Our work aimed to evaluate the influence of H on milk qualityand bioactive components and if this affects developmental and immunological parameters of offspring in early lactation. To this end, 12weeks old Wistar rats were injected with 0,25 mg/kg T4 (hyper) and vehicle (control) and euthanized on day 2 of lactation (L2). Serum,milk, and mammary gland from dams and offspring serum were obtained for further analysis. Total IgG and IgA levels from mothers andoffspring serums and rat milk were analyzed by ELISA. Nutritional components like total proteins, reducing sugars (lactose), caloric value,and fat content was measured in milk. Moreover, histological analysis of the mammary gland was performed, and offspring developmental,biochemical, and hormonal parameters were evaluated on L2. Our results show that H pups had lower weight on days 1 and 2 (p