INTERLEUKIN-6 CONCENTRATION IN SERUM AND PLACENTAL EXTRACTS DURING PORCINE GESTATION

GIAI ROMINA; WILLIAMSON, DELIA; VELEZ, CAROLINA; CLAUZURE, MARIANGELES

Buenos Aires

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020; 2020

SAI-SAIC-SAFIS

Mammalian pregnancy requires coordinated interactions between the conceptus and gestating endometrium that involves the generation of an inflammatory immune response. In earlier stages after implantation, this local inflammatory immune response produces an anti-inflammatory response, that includes numerous cytokines that regulate the intrauterine and systemic environment. This study aimed to evaluate the profile expression of IL-6 during porcine gestation. Swine placentas and sera were collected at 32, 44, 50, 60, 70, 80, 90, 114 days of gestation (dg) from slaughterhouses near General Pico, La Pampa, Argentina. Animals were sacrificed according to the animal welfare manual of National Agrifood Health and Quality Service (SENASA). The placentas were separated in fetal and maternal portions to obtain homogenates of the fetal placenta (HoFP) and maternal placenta (HoMP). As a control, we prepared homogenates from non-pregnant uteri (HoU). IL-6 concentration was determined by a specific porcine ELISA kit (R&D Systems, USA). In this first approach of this study, it was observed that this cytokine is elevated at 32 dg in HoFP (878.29 pg/ml). Around 32 days of gestation begins the ossification of the embryos and the stage of the exponential growth of the placenta. After this peak, its concentration decreased reaching basal values (below 37 pg/ml), which are similar concentrations than that determined in HoU (34 pg/ml). On the other hand, analyzing the results in HoMP, we found a markedly increasing of IL-6 at 50-80 dg: 50 (48.4 pg/ml), 60 (126.74 pg/ml), 70 (66 pg/ml), and 80 dg (87 pg/ml). In this period occurs the end of placental development and the beginning of the exponential growth of fetuses. Finally, we found a pattern of two biphasic curves of IL-6 expression. The first corresponds between 44-50 dg and the second correspond to the 70-90 dg, reaching a maximum value at 90 (384.85 pg/ml). Interestingly, we observed a basal value in serum at 60 dg which is the same period in that we found the maximum value for IL-6 in HoMP. As a preliminary conclusion, the variability of the IL-6 expression observed, suggest that this cytokine could be necessary in the molecular events that occur at placental remodeling during gestation.