Concentration of IL-6, IL-12 and IFN-?¡Ñ in serum and placental extracts during porcine gestation.

KONCURAT, MIRTA; WILLIAMSON, DELIA; VELEZ, CAROLINA; RIESCO, OSCAR

Buenos Aires

Congreso; FIRST FRENCH-ARGENTINE IMMUNOLOGY CONGRESS; 2010

SOCIEDAD ARGENTINA DE INMUNOLOGIA, SOCIEDAD ARGENTINA DE PEDIATRIA, ETC

Mammalian pregnancy requires coordinated interactions between conceptus and gestating endometrium which involve several hormones, growth factors and cytokines that act through specific receptors. Sows have an epitheliochorial, diffuse, adecidua, folded and non-invasive placenta. It is a natural target of the immune system because of its location as a barrier between mother and embryos. The aim of this study was to investigate the development of pig pregnancy through the expression of IL-6, IL-12 and IFN-?× in sera and in porcine placental extracts of different gestational periods. Pig placentas and sera were collected (n=40) at 5, 15, 20, 32, 44, 51, 60, 70, 80, 90, 114 Days of pregnancy (at term). The placenta were carefully separated in fetal and maternal portions to form placental extracts called homogenates of fetal placenta (HoFP) and maternal placenta (HoMP). Determination of cytokines by a commercial ELISA kit is carried out. Values found on day 32 of pregnancy in HoFP from IL-6 (878.29 pg/ml), IL-12 (203.90 pg/ml) and IFN-?× (505.024 pg/ml) in relation to those dosed in serum (p