Dual effects of Arachidonic acid on BK channel: role of B1-subunit

MONCADA, M; PICCININI, N; CASTILLO, K; ASUAJE, A; GONZÁLEZ, C; MILESI, V; MARTÍN, P

Sierra de la Ventana

Congreso; XLIII Reunión Anual de la Sociedad Argentina de Biofísica; 2014

Sociedad Argentina de Biofísica

Arachidonic acid (AA) is a polyunsaturated fatty acid involved in modulation of several ion channels activity. Previously, we reported that 10 μM AA activates the high conductance Ca2+ and voltage dependent K+ channel (BK) in human vascular smooth muscle cells where the alfa subunit of BK is expressed together with the regulatory B1-subunit (B1) [1]. In this work, we studied in depth the action mechanism of AA using patch clamp technique on BK channel heterologously expressed with or without B1. 10 μM AA activated BK only in presence of B1, changing the voltage dependence of activation (left shift on G-V curve, deltaV1/2= -55,2 mV ± 4,4; n=3; p