Colitis inducida por TNBS en ratones C57Bl/6como modelo para estudiar el rol de Galectinas y microbiota en enfermedades inflamatorias intestinales

MOROSI, LUCIANO; MORALES, ROSA; TOSCANO, MARTA A; BLANCATO, VICTOR; MAGNI, CHRISTIAN; RABINOVICH, GABRIEL A.; MARIÑO, KARINA V.

Tucuman

Otro; Mecanismos Moleculares y celulares inolucrados en la interacción huésped hospedador: bases para el desarrollo biotecnológico de nuevos productos con aplicación en salud; 2014

CERELA-CONICET

Our laboratories investigate the role of glycans and their interaction with endogenous lectins in the development and resolution of chronic inflammatory diseases, with a particular interest in IBD. Galectin-1 (Gal-1), a glycan- binding protein that recognizes N- and O-glycans bearing the disaccharide N-acetyllactosamine (Galβ(1-4)- GlcNAc) exerts protective and immunomodulatory activity in TNBS-induced colitis (Santucci et al., 2003). With the overarching goal of studying the role of endogenous Gal-1 in mucosal homeostasis and intestinal immunoregulation and validating its therapeutic activity in IBD, we optimized a model of 2,4,6- Trinitrobenzenesulfonic acid (TNBS)-induced colitis and evaluated the clinical outcome of wild-type and Gal-1 knock-out (Lgals1-/-) mice in the C57BL/6 background. Validation of the anti-inflammatory activity of Gal-1 in this model may allow the examination of cell surface glycosylation in cellular compartments of the mucosal microenvironment and the identification of Gal-1 function, revealing a potential target for the treatment of human IBD. Furthemore, future studies will be also focused in the role of different microbiota in the development of IBD.Assessment of the clinical score of TNBS-instilled mice revealed increased clinical severity in Lgals1-/- compared to WT mice, as shown by total weight loss and colon weight/length ratio (p