EXPLORING THE ROLE OF THE GALECTIN-1-GLYCAN AXIS IN AN EXPERIMENTAL MODEL OF COLITIS- ASSOCIATED COLORECTAL CANCER

MERLO, JOAQUÍN P.; CAGNONI, ALEJANDRO J.; CUTINE, ANABELA M.; RABINOVICH, GABRIEL A.; MARIÑO, KARINA V.

Virtual

Congreso; Reunion de Biociencias 2021; 2021

SAIC, SAI

Objective. To understand the mechanisms underlying the protumorigenic role of Galectin-1 (Gal1) and associated glycans in colitis-associated colorectal cancer (CACRC).Materials & Methods. We performed transcriptomic analysis of tumor samples from WT and Gal1 KO mice in an experimental model of CACRC (azoxymethane/dextran sulfate sodium, AOM-DSS). Total RNA was extracted, purified, and sequenced. Sequencing data waspre-processed with FastQC and Trimmomatic, and pseudo-counts were estimated using Kallisto. Downstream analyses were run using R software v3.6. Immune infiltrate deconvolution was performed using MIXTURE with ImmuCC-seq signature. Enrichment analyses were performed using GSEAbase package. KO vs. WT comparisons were performed using Wilcoxon test.Results. We recently reported the immunosuppressive role of Gal1 in AOM-DSS through modulation of the CD8+ regulatory T cell compartment, with significantly decreased tumor number and volume in Gal1 KO mice. Here, we performed transcriptomics analysis inWT and Gal1 KO mice treated with AOM-DSS. Differential expression analysis and functional enrichment showed downregulation of angiogenesis-associated pathways (p