Carrier in carrier: catanionic vesicles based on amphiphilic cyclodextrins complexed with DNA as nanocarriers of doxorubicin

ALARCÓN LILIANA P.; HEBER E. ANDRADA; OLIVERA MARÍA E; SILVA FERNANDO OSCAR; DARÍO FALCONE

JOURNAL OF MOLECULAR LIQUIDS

ELSEVIER SCIENCE BV

Año: 2022

0167-7322

Doxorubicin (Dox) is a very effective chemotherapeutic agent; however, it caninduce various side effects on normal tissues because of their non-specific distribution inthe body. Therefore, there is an urgent demand to develop systems to successfully carrydrugs to the desired sites of therapeutic action while reducing side effects. The aim of thisstudy was to evaluate the interaction between catanionic vesicles based on amphiphiliccyclodextrins (ModCBHD) and DNA?Dox complex as a supramolecular anticancerdrugs carrier (ModCBHD-DNA-Dox). The complexes were characterized by dynamiclight scattering (DLS), zeta potential (ζ), circular dichroism (CD), emission spectroscopy,atomic force microscope (AFM), transmission electron microscopy (TEM). The resultsshowed that ModCBHD vesicles exhibit a positive surface charge that allows negativelycharged DNA-Dox to wrap them. Besides, Dox interacts with DNA through intercalationof the tetracene ring system and by ionic interaction through the amino sugar residue ofthe drug. In addition, ModCBHD-DNA-Dox exhibit a small particle size and optimalmonodisperse size distribution that can take advantage of the enhanced permeability andretention (EPR) effect in cancer therapy.In vitro release of Dox from ModCBHD-DNA-Dox was slower than from DNA?Dox, due to the increasing appearance of external complexes formed from partiallyintercalated Dox after all the available sites become occupied upon addition ofModCBHD.The study overall highlighted a novel strategy that combine vesicles preparedfrom amphiphilic cyclodextrins and DNA that can be used as alternative carriers forchemotherapeutic agents such as Dox with slow-release properties.