PRELIMINARY STUDY ON PREVALENCE OF CLASS 1 INTEGRONS AND EXTENDED-SPECTRUM β-LACTAMASES (ESBL) IN CLINICAL ISOLATES FROM MENDOZA HOSPITALS, AND TUNE-UP TOOLS FOR SURVEILLANCE OF INTRAHOSPITAL INFECTIONS

MARQUEZ F; GARAY C; NOLLY M; LEIVA N; SÁNCHEZ D; DAMIANI MT

Mendoza

Congreso; XXXVI REUNIÓN CIENTIFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2018

PRELIMINARY STUDY ON PREVALENCE OF CLASS 1 INTEGRONS AND EXTENDED-SPECTRUM β-LACTAMASES (ESBL) IN CLINICAL ISOLATES FROM MENDOZA HOSPITALS, AND TUNE-UP TOOLS FOR SURVEILLANCE OF INTRAHOSPITAL INFECTIONS Marquez F1,*. Garay C1,*, Nolly M1,3, Leiva N1,2, Sánchez D1,3, Damiani MT1,3 1Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo. 2Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Cuyo. 3IMBECU-CONICET, Mendoza. Argentina. *Ambos autores contribuyeron igual a este trabajo. E-mail: dgsanch@yahoo.com.ar The dissemination of genes for resistance to antibiotics by genetic elements, especially those related to class 1 integrons, is a growing concern worldwide due to the substantial increase of strains resistant to multiple drugs, especially those resistant to carbapenems and/or third and fourth generation cephalosporins. Resistance cassettes dissemination studies is an extremely relevant issue that concerns antibiotic administration policies. In this work, we determined the occurrence of class 1 integrons and extended-spectrum beta-lactamases (ESBL) in clinical isolates from local hospitals, using PCR techniques. On the other hand, nosocomial infections are among the most common complications in intensive care units. Bacteria are capable of surviving the adverse conditions found at the hospital environment, and the use of antibiotic pressure turns them multi-resistant to various antimicrobials. Therefore, we were interested in acquiring adequate and flexible tools to identify possible reservoirs of strains that propagate clonal infections inside the hospital. For this aim, we tune-up the Random Amplification of Polymorphic DNA (RAPDs) in order to be able to quickly identify infection focus, making possible an efficient control outbreak of clonal infection within hospitals. For this aim, we tune-up the Random Amplification of Polymorphic DNA (RAPDs) in order to be able to quickly identify infection focus of P. aeruginosa, making possible an efficient control outbreak of clonal infection within hospitals. We found that only 2 of the 4 primers tested for RAPDs were optimal for reproducibility. Also, our preliminary studies based on the identification of class 1 integron show that only 4 of the 12 strains obtained so far (33%), are positive for this integron. This rate of occurrence of class 1 integron is in accordance with the ones obtained in other countries such as the United States, Brazil and the European Union.