CONICET | Buscador de Institutos y Recursos Humanos

Bone can be considered as a biological hybrid material composed of organic andinorganic components: collagen and rod-shaped hydroxyapatite (HAP) of 20?50 nm lengths. The synthetic hydroxyapatite (Ca10(PO4)6(OH)2) has been extensively used as a bone substitute material due to its chemical and structural similarities with naturalmineral bone. One way to obtain HAP nanoparticles is by using self-assembled amphiphilic molecules as structure directors. This study involves different hexadecyltrimethylammonium bromide (CTAB) micellar-block copolymer organized networks. Inorganic precursors were added insequence to the CTAB-polymer solution, followed by a hydrothermal treatment. The final productwas separated from the suspension by filtration and then dried. The X-ray diffraction and infrared spectroscopy pattern of the materials synthesized corresponds to the HAP pattern. Transmission and scanning electron microscopy microphotographs show a fiber network composed by 37 nm length HAP nanorods. After treatment with simulated body fluid (SBF) a layer of HAP nanocrystals grew on the material surface; that is related to thebioactivity of the material. To confirm the samples´ biocompatibility, calvarial osteoblasts obtained from neonatal rats were exposed to the material and then, viability and celladhesion were evaluated. A new method of HAP nanocrystals with similar shape,morphology and chemical characteristics of bone were developed. After SBF immersion, material revealed a spherulitic-like HAP layer that implies a positive physiological response and good bond ability to the host tissue. Therefore, nanomaterials obtained by the proposed synthesis could have a wide range of biomedical applications.

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