Isolation and characterization of Pseudomonas spp. from the rhizosphere of soya with activity in vivo against corynespora cassiicola

MICHAVILA G; ADLER C; CARAM DI SANTO MC; CORBALAN NS; POMARES MF; DE CRISTOBAL RE; VINCENT PA

La Falda - Cordoba

Workshop; II Taller Latinoamericano sobre Rizobacterias Promotoras del Crecimiento Vegetal; 2014

II PGPR Latinoamérica

The ring spot disease of soya (Glycine max (L.) Merr.) caused by Corynespora cassiicola, is found in most Argentinean soybean-growing zones, although it is more common in the northern provinces. The inadequate use of agrochemical products is dangerous for the environment, so is interesting to search for effective management alternatives that do not select resistance in the pathogen and that can be produced without petrochemical industry energy use. One alternative is the use of non-synthetic, biological control agents. The bacteria that inhabit the rhizosphere and have ability to cause a positive effect on plants are called plant growth promoting Rhizobacteria (PGPR). In general, biological control mechanisms and plant growth promotion by rhizospheric bacteria are based on microbial metabolites that adversely affect the pathogen or induce systemic resistance in the plant. Our laboratory addressed a screening for rhizospheric bacteria featuring plant promoting traits and antibiotic action against plant pathogens. Pseudomonas bacteria genera was isolated from soy rhizosphere. We studied biochemical markers associated with growth promotion i.e. siderophore production, synthesis of indole acetic acid and phosphate solubilization. Preliminary in vivo assays using soy plants showed a protective effect of the Pseudomonas strain against the causal agent of ringspot disease in soy (Corynespora cassiicola). However, no antibiotic activity was observed in vitro. These results indicate a promising role of this Pseudomonas strain as a biocontrol agent for plant diseases through the induction of the plant defense response.