Genetic variability assesment using mitocondrial DNA marker for pre-breeding of pacu (Piaractus mesopotamicus).

FREITAS, M.V.; ARIEDE, R.B.; MASTROCHIRICO-FILHO, V.A.; MENDOZA, F.F.; PORTO-FORESTI, F.; VILLANOVA, G.V.; DEL PAZO, F.; HASHIMOTO, D.T.

Conferencia; VI Conferencia latinoamericana sobre cultivo de peces nativos: I Congreso Uruguayo de Acuicultura.; 2018

Resumen para la VI Conferencia latinoamericana sobre cultivo de peces nativosUsuario: Milena VieiraÁrea principal: Genética y mejoramiento genéticoÁrea alternativa: Modalidad: pósterTítulo: GENETIC VARIABILITY ASSESSMENT USING MITOCHONDRIAL DNA MARKER FOR PRE-BREEDING OF PACU (Piaractus mesopotamicus) Autor: Freitas, M.V. (1); Ariede, R.B. (1); Mastrochirico-Filho, V.A. (1); Mendonça, F.F. (2); Porto-Foresti, F. (3); Villanova, G.V. (4); Del Pazo, F. (4); Hashimoto, D.T. (1)Filiación: (1) CAUNESP, UNESP, Jaboticabal, SP.; (2) Instituto do Mar, UNIFESP, Santos, SP.; (3) UNESP, Bauru, SP.; (4) Universidad Nacional de Rosario, Rosario, Argentina. Email: milenavfreitas@gmail.comResumen: Genetic diversity in cultivated stocks is fundamental to the maintenance of genetic potential, especially when initiating fish breeding programs. Incorrect reproductive management, coupled with the use of few breeders, tends to decrease genetic variability. This results in loss of genetic potential and depression due to inbreeding. In this study, analyzes of the mitochondrial DNA control region (CR) were used to estimate the genetic diversity levels of Piaractus mesopotamicus females (n = 41 dams) from the breeding program of Caunesp (Aquaculture Center of the São Paulo State University, Brazil). Moreover, breeding stocks from eight commercial fish farms were used for comparative evaluations, four from Brazil (PB1-PB4) and four from Argentina (PA1-PA4). We sequenced around 400 bp (base pairs) that characterized 47 polymorphic sites and 51 mutations, evidencing 34 haplotypes, of which 10 were shared between fish farms and 24 are exclusive. The nucleotide diversity (π) ranged from 0.00031 to 0.01462 and haplotype diversity (Hd) from 0.125 to 0.868. The analysis of molecular variance (AMOVA) indicated high structure present in the analyzed stocks (FST = 0.13911 and ФST = 0.51813). In general, the genetic diversity was high, especially in the breeding stock from Brazilian fish farms. In relation to the breeding population of Caunesp, we observed the presence of 7 haplotypes, of which 4 were exclusive and 3 shared between the commercial fish farms. The genetic diversity was moderate (π = 0.00265 and Hd = 0.424) and considered appropriated for the pre-breeding of pacu. The unique haplotypes identified in this work may serve as traceability markers for products resulting from the genetic improvement of pacu. In conclusion, our results provided support for the genetic diversity maintenance of the pacu commercial broodstocks and will serve as framework for research with other native species of economic interest in the South America aquaculture. Financial Support: FAPESP (2016/21011-9, 2015/14185-8, 2014/03772-7), CNPq (305916/20157, 446779/2014-8) and CAPES. Fecha: 2018-08-17 15:04:35