Evaluation of the genetic diversity of microsatellite markers among four strains of Oreochromis niloticus

DIAS MARCO AURELIO DESIMONI; DE FREITAS RILKE TADEUS FONSECAS; ARRANZ SILVIA EDA; VILLANOVA GABRIELA VANINA; HILSDORF ALEXANDRE

ANIMAL GENETICS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2016 vol. 47 p. 345 - 353

0268-9146

Different strains of Nile tilapia can be found worldwide. To successfully use them in breeding programs, they must be genetically characterized. In this present study, four strains of Nile tilapia?UFLA, GIFT, Chitralada, and Red-Stirling?were genetically characterized using 10 noncoding microsatellite loci and two microsatellites located in the promoter and first intron of the growth hormone (GH) gene. The two microsatellites in the GH gene were identified at positions ?693 to ?679 in the promoter [motif (ATTCT) 8] and in intron-1 at position +140 to +168 [motif (CTGT)7]. Genetic diversity was measured as mean numbers of alleles and expected heterozygosity, which were 4 45 and 0.60 (GIFT), 3.5 and 0.71 (UFLA), 4.5 and 0.57 (Chitralada), and 2.5 and 0.42 (Red-Stirling), respectively. Genetic differentiation was estimated both separately and in combination for noncoding and GH microsatellites markers using Jost?s DEST index. The UFLA and GIFT strains were the least genetically divergent (DEST = 0.10), and Chitralada and Red-Stirling were the most (DEST = 0.58). The UFLA strain was genetically characterized for the first time and, because of its unique origin and genetic distinctness, may prove an important resource for genetic improvement of Nile tilapia. This current study shows that polymorphisms found in coding gene regions might be useful for assessing genetic differentiation between strains.