Genotoxicity in Caiman latirostris hatchlings exposed in vivo to two commercial formulations of glyphosate, through the Micronucleus test and nuclear abnormalities.

LÓPEZ GONZÁLEZ EVELYN C.; LARRIERA ALEJANDRO; SIROSKI, PABLO A.; POLETTA GISELA L.

Asunción

Otro; XVIII Curso de Posgrado Internacional Alexander Hollaender: sobre Genética Toxicológica, Mutagénesis, Carcinogénesis y Teratogénesis Ambiental; 2013

Sociedad Paraguayana de Mutagénesis, Carcinogénesis y Teratogénesis ambiental

Genotoxicity in Caiman latirostris hatchlings exposed in vivo to two commercial formulations ofglyphosate, through the Micronucleustest and nuclear abnormalities. López González, Evelyn C.1,2; Larriera, Alejandro1, Siroski, Pablo A.1,2,3; Poletta, Gisela L.1,2,4   1??Proyecto Yacaré??- Lab. Zoología Aplicada: Anexo Vertebrados (FHUC-UNL/MASPyMA). Av. Aristóbulo del Valle 8700 (3000), Santa Fe, 342-4-579256. 2CONICET. Av Rivadavia 1917 (C1033AAJ), CABA. 3Instituto de Ciencias Veterinarias del Litoral (ICiVet-CONICET), Esperanza, Santa Fe. 4Cát. Toxicol. y Bioq. Legal, FBCB-UNL. Ciudad Universitaria - Paraje El Pozo S/N (3000), Santa Fe. evelynclg@hotmail.com   Caiman latirostris is one of the two crocodilian species living in Argentina. As a result of agricultural expansion produced in recent years, some populations are exposed to continuous pesticide discharge due to overlapingwith extensive agricultural areas. The aim of this study was to evaluate genotoxicity of two commercial formulations of glyphosate (Roundup® -RU, 66,2%- and PanzerGold® -PANZ, 60,2%) on C. latirostris hatchlings. This study was carried out in Proyecto Yacaré (Gob. Santa Fe/MUPCN) facilities. C. latirostris hatchlings, 20 days old, were exposed to two concentrations of RU (RU1 and RU2) and two of PANZ (PANZ1 and PANZ2), including the ones recommended for their application in the crops, and a control without treatment (NC). Animals were maintained in plastics pens, during two months and concentration of pesticides progressively decreased through time in order to simulate their degradation in natural conditions. At the end of exposure period, blood samples were obtained and the Frequency of micronucleus (FMN) and nuclear abnormalities (FNA) determined in erythrocytes as markers of genotoxicity. Nuclear abnormalities included: buds, binuclei, excentric nuclei, notched nuclei and vacuolated nuclei. Up to this moment results indicated a significant increase in the FMN in the groups exposed to the lower concentration of Roundup® (RU1) and to the higher of PanzerGold® (PANZ2) compared to the NC (P<0.05). No significant different was observed in the FNA in any of the exposed group, respect to the NC. The MN test showed to be more sensitive to detect genotoxic effect of these pesticides than the FNA. These results, together with those reported in previous studies; warn about the effect that C. latirostris wild populations continuously exposed to low concentrations of pesticides might be suffering.