Rapid identification of Burkholderia cepacia complex species including strains of the novel Taxon K, recovered from cystic fibrosis patients by intact cell MALDI-ToF mass spectrometry (ARTICULO DISTINGUIDO - The Analyst)

ALEJANDRO MIÑAN; ALEJANDRA BOSCH; PETER LASCH; MAREM STÄMMLER; DIEGO O. SERRA; JOSÉ DEGROSSI; BLANCA GATTI; CARLOS VAY; MIGUEL D'AQUINO; OSVALDO YANTORNO; DIETER NAUMANN

ANALYST

ROYAL SOC CHEMISTRY

Lugar: Cambridge ; Año: 2009 p. 1138 - 1148

0003-2654

ESTE TRABAJO FUE DISTINGUIDO COMO "HOT ARTICLE" POR LA REVISTA "THE ANALYST". RESUMEN: Two approaches based on intact cell matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (IC-MALDI-ToF MS) have been evaluated in order to discriminate and identify nine former Burkholderia cepacia complex (Bcc) species, Burkholderia contaminans belonging to the novel Taxon K, Burkholderia gladioli, and the most relevant non-fermentative (NF) Gram-negative rods recovered from cystic fibrosis (CF) sputum cultures. In total, 146 clinical isolates and 26 reference strains were analysed. IC mass spectra were obtained with high reproducibility applying a recently developed inactivation protocol which is based on the extraction of microbial proteins by trifluoroacetic acid (TFA). In a first approach, spectral analysis was carried out by means of a gel-view representation of mass spectra, which turned out to be useful to recognize specific identifying biomarker proteins (SIBPs). A series of prominent mass peaks, mainly assigned to constitutively expressed proteins, were selected as SIBPs for identifications at the genus and species level. Two distinctive mass peaks present in B. contaminans spectra (7501 and 7900 Da) were proposed as SIBPs for the identification of this novel species. A second approach of spectral analysis based on data reduction, feature selection and subsequent hierarchical cluster analysis was used to obtain an objective discrimination of all species analysed. Both complementary modalities of analyzing complex ICMALDI- ToF MS data open the path towards a rapid, accurate and objective means of routine clinical microbiology diagnosis of pathogens from sputum samples of CF patients. CARTA AL CORRESPONING AUTHOR. Dear Professor Naumann.Paper  Ref.: B822669E. Title : Rapid identification of Burkholderia cepacia Complex species including strains of the novel Taxon K, recovered from cystic fibrosis patients by intact cell MALDI-ToF mass spectrometry. I am very pleased to tell you that your manuscript has been selected as an Analyst Hot Article. It is currently featured in the Hot Articles section of our website at http://www.rsc.org/Publishing/Journals/an/HotArticles.asp and will be free to access for four weeks.I would like to take this opportunity to thank you for choosing to publish your work in Analyst and to invite you to submit your next research article for publication in our journal. We look forward to receiving your next paper soon. With best wishes Dr Niamh O´Connor. MRSC, Editor, Royal Society of Chemistry,Thomas Graham House, Science Park , Milton Road, Cambridge , UK