? VITAMIN C (VitC) REVERTS INTESTINAL Mrp2 DOWN-REGULATION IN RATS WITH FRUCTOSE INDUCED METABOLIC SYNDROME (MS).

ZECCHINATI F; TOCCHETTI GN; DOMINGUEZ CJ; BARRANCO MM; ARANA MR; PERDOMO VG; LUQUITA, MG; MOTTINO AD; GARCÍA F; VILLANUEVA SSM

Rosario

Congreso; REUNIÓN ANUAL SAFIS 2019; 2019

Sociedad Argentina de Fisiología

Intestinal Mrp2 is an ABC transporter that limits the absorption of xenobiotics orally ingested, thus acting as a biochemical barrier. MS is a pathological condition characterized by insulin resistance, hyperinsulinemia and dyslipidemia as well as by chronic inflammation and oxidative stress (OS). In previous studies we observed that MS-like conditions induced by fructose in drinking water (10% v/v, during 21 days), reduced the expression and activity of intestinal Mrp2 in rats. We here evaluated the effect of VitC (100 mg/kg/day; p.o), a natural potent antioxidant, in reverting fructose-induced Mrp2 alterations. Once the MS features were settled, animals were cotreated with VitC for another 14 days. VitC reverted down-regulation of Mrp2 protein induced by MS, as detected by western blotting (C: 100%, FRU: 25%, FRU+VitC: 102%, P