Histopathological, Inmunohistochemical and Biological Characterization of the CFM-27 Tumoral Line obtained by Syngeneic Passages

LOMBARDO, DM; FIORITO, C; QUIROGA, S; DE SANDE, C; VARANI, A; BUSTOS, R; FORASTIERS, N

BIOCELL

INST HISTOL EMBRIOL-CONICET

Año: 2006 vol. 30 p. 516 - 516

0327-9545

Tumors obtained by induction in Wistar rats with n´nitroso n´methyl urea (NMU), demonstrated an histopathological pattern corresponding to an alveolar mammary adenocarcinoma. Those originated in mice only treated with medroxiprogesterone (MPA) were ductal mammary carcinomas. Inductions with NMU in mice Balb/c did not reproduce the tumorigenic effect. The induction and later promotion with MPA in mice Balb/c generated an alveolar mammary adenocarcinoma. After several syngeneic passages of tumoral fragments obtained by induction and promotion a tumoral line with different histopathologic character was stablished. Mice of 5 weeks were induced with three doses of NMU (50 mg/kg) and three doses of MPA (40 mg). Two months after induction tumors were developed and detectables by palpation. These were transplanted in syngeneic form to female mice without inmunosuppression. They were carried out 42 successive passages in Vivo and samples were frozen at -120ºC to repeat passages starting from the same one. The transplants were in the inguinal pleat. With a latency of 7 days they were developed tumors locally, differing from the original pattern to a sarcoma of small cells. With the histological routine Tricrhomics were carried out and the cellular pattern was determined by inmunohistochemistry, using membrane markers like Cadherin E, N, Cytoqueratin P8 and Pan cytoqueratine. The expression of receptor of hormon-dependence was evaluated with Progesterone monoclonals anti receptor of and anti protein P29. Up to now the CFM-27 line maintains its histopathologic pattern without changes in successive passages, being able to be a tumoral model repetible for the study of different biological aspects of neoplasia.