DEXAMETHASONE INHIBITS BOTH RETROPERITONEAL AND SUBCUTANEOUS WHITE ADIPOSE TISSUE BROWNING

GIORDANO, A; ZUBIRÍA, M.G.; HARNICHAR, EA; CASTRO, P; GIOVAMBATTISTA, A.

Congreso; la Reunión Anual de Sociedades de Biociencias 2022- SAIC; 2022

Two mechanisms are described for browning of White Adipose Tissue (WAT): trans-differentiation from white to beige adipocytes or Adipocyte Precursor Cells (APCs) differentiation. Glucocorticoids (GCs) inhibit WAT browning, but remains unknown if they affect mature beige adipocytes, APCs or both. Our aim was to study Dexamethasone (DXM) effect on browning markers in whole WAT and in isolated Stromal Vascular Fraction (SVF) cells from Retroperitoneal and Inguinal AT (RPAT and IAT, respectively). Male rats were divided into four groups: control (CTR) and DXM (sc. Injected, 0,03mg/Kg/d for 7 days) housed at room temperature (RT), and CTR and DXM housed under cold (C) stimulus (4°C for 7 days, CTR-C and DXM-C, respectively). Two-way ANOVA test was used for statistical analysis. Firstly, we evaluated the DXM effect in whole WAT. RPAT and IAT pads were dissected and processed for quantification of thermogenic genes (qRT-PCR). We found that DXM inhibited the expression of different thermogenic markers. In RPAT, Ucp-1, pgc1a and dio2 levels was lower in C than RT (Interaction DXMxC p