EFFECT OF KETAMINE ON EXOCYTIC AND ENDOCYTIC MACHINERY IN RAT NUCLEUS ACCUMBENS

RUIZ, AM.; CARVELLI, L.; TRONCOSO, M.; GIL LORENZO, A.; CASAS, S.; ESCUDERO, C.; CABRERA, R.; SOSA, MA.

San Luis

Congreso; XXX Reunión ciéntifica anual de la Sociedad de Biología de Cuyo. San Luis. Año 2012.; 2012

Sociedad de Biología de Cuyo

Clathrin-mediated endocytosis (CME) is important in the central nervous system for maintainance of membranous homeostasis at nerve terminals. Ketamine (K) is a NMDA receptor blocker. We wondered if the drug affects the expression and cycle of coat proteins involved in CME in the rat nucleus accumbens (NA) and if it also induces alterations in exocytic machinery. Adult rats were treated with subanesthetic doses of K for 15 min, 3 h or 24 h. The NA were dissected, homogenized and centrifuged to obtain membranous and soluble fractions. The coat protein AP-2 was analyzed in both fractions by Western blot. We observed increased expression of AP-2 protein compared to non treated controls. In order to determine whether K affects exocytosis, the NA were subject to the superfusion method using [3 H] dopamine. The release of [3 H] dopamine was measured and referred to basal values of controls. It was observed that release of [3 H] dopamine decreased from 15 min of K treatment. After 24 h, this effect was mitigated. These results suggest an acute effect of K on the endocytic/exocytic pathway in the nucleus accumbens