UHPLC-Q/Orbitrap/MS/MS fingerprinting and antitumoral effects of Prosopis strombulifera (LAM.) BENTH. aqueous extract on allograft colorectal and melanoma cancer models. *Equal contribution

PERSIA, FA *; TRONCOSO, M * ; RINALDINI, E; SIMIRGIOTIS, M.; TAPIA, A.; BORQUEZ, J.; MACKERN, JP; HAPON, MB; GAMARRA LUQUES, C

Heliyon

Elsevier

Año: 2020 vol. 6

2405-8440

The aqueous extract of the Argentinean native plant, Prosopis strombulifera (PsAE), presents cytotoxicity against human cancer cell lines by inducing cytostasis, necrosis and apoptosis; with significant diminution of clonogenic survival; without genotoxic effects nor animal toxicity when it is orally administrated. However, the chemical extract composition and its antitumoral properties in vivo remain unknown. In consequence, these studies are the aim of the current work. The PsAE was characterized by chemical fingerprinting and the metabolome was identified by tandem UHPLC-PDAHESI-Q-orbitrap® mass spectrometry. Colorectal tumors were induced by DMH administration and melanomas resulted from B16-F0 S.C. cells injection; then, animals were treated orally with PsEA. To correlate in vivo results with in vitro cytotoxicity, were determined: cell proliferation and viability, cell cycle distribution, and p21cip1, PCNA,cleaved caspase 3 and cleaved PARP expression by dye exclusion assays, flowcytometry and immunoblotting, respectively. Based on UHPLC-OT-MS and PDAanalysis, twenty-six compounds were identified in the PsAE; including: five simple organic acids, four phenolic acids, four procyanidins, eleven flavonoids, and two oxylipins. On C57BL6 mice, PsAE significantly increases the median survival on colorectal cancer and reduces the final volume and weight of subcutaneous melanomas. Over cultured B16-F0 cells, the treatment induce over-expression of p21, cytostasis by G2/M cell cycle arrest and apoptosis; while, on in vivo melanomas, treatment up-regulates p21 and slightly decreases PCNA. In conclusion, PsAE is composed by phenolic compounds which demonstrate cytotoxic and antitumoral properties when is orally administrated. Presented results support future research of PsAE as a potential phytomedicine for cancer treatment.