Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus

HERNÁNDEZ CHRISTIAN A.; SANDOVAL NORMA; MALLERMAN JULIETA; GARCÍA-PÉREZ JOSÉ ANTONIO; FARNET ANNE-MARIE; PERRAUD-GAIME ISABELLE ; ALARCÓN ENRIQUE

ELECTRONIC JOURNAL OF BIOTECHNOLOGY

UNIV CATOLICA DE VALPARAISO

Lugar: Valparaíso; Año: 2015 vol. 18 p. 327 - 332

0717-3458

Background: Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results: In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L)induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance withthe surface response model, as long as simple nitrogen sources (urea) were not available. Conclusions: We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repression mechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production.