THE ω3 SUPPLEMENTATION INHIBITS THE DEVELOPMENT OF THE SLOW FORCE RESPONSE IN OVARIECTOMIZED RATS

GARCÍA RB; ZAVALA MR; DIAZ, RG; PINILLA OA; VELEZ RUEDA, JO; LOFEUDO, JM; PEREZ, NG; DE GIUSTI, VC; VILLA-ABRILLE MCM

Buenos Aires

Congreso; Congreso Anual SAFIS 2023; 2023

SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS)

IntroductionMyocardial stretch induces a rapid increase in force, followed by the slow force response (SFR). The production of stretch-induced reactive oxygen species (ROS) is crucial for its development and the activation of cardiac Na+/H+ exchanger (NHE1). Many studies describe that the consumption of polyunsaturated fatty acids of the omega-3 (ω3) series has cardioprotective effects. We recently demonstrated that ovariectomized (OVX) rats have increased expression and NHE1activity.ObjectivesCharacterize the SFR development in OVX rats supplemented with ω3.Methods Two-month-old female rats were randomized into two groups: Sham (S) and bilaterally ovariectomized (OVX). One month after surgery, a group of OVX received 200 mg/kg/day ω3 orally, for 3 months (OVX ω3). Echocardiographic parameters and systolic blood pressure (SBP) were determined in the all protocol. After 3 months, the development of SFR in isolated papillary muscles, the ROS production and NHE1 expression in left ventricular slices were evaluated. ResultsOvariectomy produced uterine atrophy and body weight increase without affecting SBP. The ω3 did not modify these parameters.Left ventricle mass index (LVMI) was higher in OVX (mg/mm: 20.21 ± 1.0, n= 13, p