Salinity Effect on Oxidative Stress Parameters in Tall Wheatgrass Half-Sib Families

MACIEL MARIA; TOMASSINO EZEQUIEL; ANDRES ADRIANA; GRUNBERG KARINA

Buenos Aires

Simposio; 5 th International Symposium of Forage Breeding; 2015

facultad de agronomia-Universidad de Buenos Aires

Tall wheatgrass is a valuable forage resource to use in marginal environments with saline soils. Salinity is one of the most severe problems in agricultural production. One of the main consequences of exposure to salinity, at cell level, is the overproduction of reactive oxygen species that may cause oxidative damage to macromolecules. Malondialdehide (MDA) is a by-product of membrane lipids peroxidation, and an increase in its content indicates oxidative damage. To mitigate this damage, cells have developed complex enzymatic and non-enzymatic antioxidant systems. A key enzyme in the antioxidant defense is the superoxide dismutase (SOD) that catalizes the dismutation of superoxide into oxygen and hydrogen peroxide. The aim of this study was to assess the salinity effect on oxidative stress parameters in Tall Wheatgrass Half-Sib Families (HSF). Twelve HSF were assessed in hydroponics greenhouse conditions with two treatments: C=0mM and S=150mM NaCl in a randomized completed blocks design with three repetitions (six plants/HSF/treatment). Twenty four hours after the final concentration was reached, leaf samples to determine MDA content and SOD activity were collected. At the end of the experiment, dry weight of aerial part per plant (DW) was measured. All data were expressed as ratio of values obtained in salt regarding the control mean, and were analyzed by the procedure of general linear mixed models of Infostat statistics software. Means were compared by LSD test. Significant differences (p