INVESTIGADORES
MENCHON Silvia Adriana
artículos
Título:
APM_GUI: analyzing particle movement on the cell membrane and determining confinement
Autor/es:
MENCHÓN, SILVIA ADRIANA; MARTÍN, MAURICIO; DOTTI, CARLOS
Revista:
BMC Biophysics
Editorial:
BMC BioMed Central
Referencias:
Año: 2012 vol. 5 p. 1 - 10
ISSN:
2046-1682
Resumen:
Background: Single-particle tracking is a powerful tool for tracking individual particles with
high precision. It provides useful information that allows the study of diffusion
properties as well as the dynamics of movement. Changes in particle movement behavior,
such as transitions between Brownian motion and temporary confinement, can reveal
interesting biophysical interactions. Although useful applications exist to determine
the paths of individual particles, only a few software implementations are available
to analyze these data, and these implementations are generally not user-friendly and
do not have a graphical interface. Results: Here, we present APM_GUI (Analyzing Particle Movement), which is a MatLab-implemented
application with a Graphical User Interface. This user-friendly application detects
confined movement considering non-random confinement when a particle remains in a
region longer than a Brownian diffusant would remain. In addition, APM_GUI exports
the results, which allows users to analyze this information using software that they
are familiar with. Conclusions: APM_GUI provides an open-source tool that quantifies diffusion coefficients and determines
whether trajectories have non-random confinements. It also offers a simple and user-friendly
tool that can be used by individuals without programming skills.